This product (s) resides on a Fisher Scientific GSA or VA contract. Bis Tris gels are polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. You can also view your contract price by searching for this item(s) on GSA Advantage. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Edit this message by logging in to your account, Note: Product prices and availability are subject to change without notice. ZERO BIAS - scores, article reviews, protocol conditions and more Please sign in to view account pricing and product availability. Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. A Mini Gel Tank is required for use of all Bolt gels. Use the right buffer to optimize protein separations Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. To place an order, contact Fisher Scientific Customer Service. MES SDS running buffer: 50 mM MES, 50 mM Tris base, 0.1% SDS, 1 mM EDTA, pH 7.3 Recipe for 20X buffer stock: MES 97.6 g Tris base 60.6 g SDS 10 g EDTA 3.0 g Deionized water to 500 mL Do not use acid or base to adjust pH. However, we are committed to improving your shopping experience. Bolt Bis-Tris Plus gels also come in … In the Laemmli system (Tris-glycine), the gel is run at basic pH (pH ~9.5). Fisher Scientific is always working to improve our content for you. Tricine SDS running buffer: 100 mM Tris base, All contact information provided shall also be maintained in accordance with our A Mini Gel Tank is required for use of all Bolt™ gels. Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. During separation, the gels operate close to pH 7. : Bolt™ Bis-Tris Plus gels come in four gel types and multiple well formats. We appreciate your feedback. We will not share your information for any other purposes. Shelf-life is 6 months, when stored properly. b. It is recommended for separating small- to medium-sized proteins. Bioz Stars score: 94/100, based on 3 PubMed citations. Remove the comb, and rinse the gel wells three times using 1X Running Buffer. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. The same buffer type must be used for both chambers. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Invitrogen™ 20X Bolt™ MES SDS Running Buffer. Neutral-pH buffers in NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels deliver sharp straight bands. 3 Prepare gel a. It is recommended for separating small- to medium-sized proteins. Running Buffer Standard Run The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. About Bolt™ Bis-Tris Plus Gels Novex™ 20X Bolt™ MES SDS Running Buffer 13246499 Print Novex™ 20X Bolt™ MES SDS Running Buffer Brand: Novex™ B000202 Code : 50.
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